Discussions

Competitive inhibition ELISA is also called blocking ELISA. Its main principle is to use the antigen or antibody to be tested to interfere with the pre-designed system. The final color development result is negatively correlated with the degree of interference of the antigen or antibody to be tested. Competitive inhibition ELISA is very flexible, and more complex experimental plans can be designed on this basis, thus deriving special ELISA methods such as direct competitive inhibition ELISA, indirect competitive inhibition ELISA, and sandwich competition ELISA. Here we only briefly explain the principle by taking direct competitive inhibition ELISA and indirect competitive inhibition ELISA as examples.