Discussions

Single-cell CITE-seq (Cellular Indexing of Transcriptomes and Epitopes by sequencing) is an innovative technique that combines single-cell RNA sequencing (scRNA-seq) with protein marker analysis. This approach enables multi-omic profiling at the single-cell level, offering simultaneous insights into gene expression and protein phenotype.

Key Features of Single-Cell CITE-Seq
Dual Modality: It integrates transcriptomic data with protein expression data, providing a comprehensive view of cellular states and functions.

Antibody-Derived Tags (ADTs): In CITE-seq, antibodies conjugated with unique DNA oligonucleotide barcodes are used. These barcodes are sequenced alongside the RNA, quantifying protein abundance.

High Throughput: The technique allows for the simultaneous analysis of thousands of cells, making it suitable for studying diverse and complex cell populations.

Compatibility: CITE-seq is compatible with existing single-cell sequencing platforms, facilitating its integration into established workflows.

Applications in Research
Immune Profiling: CITE-seq is particularly useful in immunology for detailed profiling of immune cell populations, distinguishing subtle differences in protein expression that may not be evident at the RNA level alone.

Cancer Research: This technology aids in characterizing tumor heterogeneity and understanding the tumor microenvironment by linking gene expression with cell surface markers.

Developmental Biology: It provides insights into developmental processes by identifying rare cell types and states based on combined transcriptomic and proteomic data.

Drug Discovery and Development: CITE-seq helps in identifying drug targets and understanding drug mechanisms by providing a complete picture of cellular responses at the molecular level.